Kaiju No. 8: Mission Recon does not explicitly portray LGBTQ+ characters or themes. The film's narrative centers on action, sci-fi elements, and the protagonist's journey of transformation and battling Kaiju, without addressing sexual orientation or gender identity within its plot or character interactions.

Kaiju No. 8: Mission Recon does not feature any identifiable transsexual characters or explore themes related to the transsexual community. The narrative centers on a defense force combating kaiju monsters, emphasizing action, teamwork, and personal growth without addressing gender identity topics.

Female characters, including Mina Ashiro, are depicted engaging in physical combat using melee weapons and hand-to-hand techniques. Their opponents are exclusively kaiju monsters, not male human characters. The film focuses on large-scale battles against these creatures, with no documented instances of female characters defeating male human opponents in direct physical combat.

The film's characters, including Kafka Hibino, Mina Ashiro, and Kikoru Shinomiya, maintain their canonical genders from the original manga and anime source material. There are no instances where a character's on-screen gender differs from their established gender in the source.

The film's animated characters and on-screen population maintain their original Japanese ethnicity and East Asian phenotypes, consistent with the source material. While the English voice cast includes actors of diverse ethnicities, this does not alter the visual racial portrayal of the characters on screen, thus no race swap occurs.