The movie Kaiju No. 8: Mission Recon does not explicitly depict or emphasize LGBTQ+ characters or themes within its narrative. It primarily focuses on action, kaiju battles, and the protagonist's journey in the Defense Force, without making LGBTQ+ identity a focal point, resulting in no identifiable representation in the film itself.

Kaiju No. 8: Mission Recon does not feature any identifiable transsexual characters or themes. The narrative centers on Kafka Hibino's transformation into a kaiju and his efforts to join the Defense Force, a plot entirely unrelated to gender or sexual identity. Therefore, the film has no depiction of transsexual individuals or related themes.

The film's characters, including Kafka Hibino, Mina Ashiro, and Kikoru Shinomiya, maintain their canonical genders from the original manga and anime source material. There are no instances where a character's on-screen gender differs from their established gender in the source.

The film's animated characters and on-screen population maintain their original Japanese ethnicity and East Asian phenotypes, consistent with the source material. While the English voice cast includes actors of diverse ethnicities, this does not alter the visual racial portrayal of the characters on screen, thus no race swap occurs.