The film includes established 'Okama Bar' characters who serve as strong, capable allies in the final battle. While their presence is supportive, their identities are not deeply explored or affirmed, nor are they subjected to new mockery within this specific film. Their portrayal is present but incidental, resulting in a neutral net impact.

The film features Catherine, an established transsexual character from the Gintama series, in a supporting role. Her trans identity is not a central theme or source of conflict within the movie's narrative. She is depicted as a loyal ally participating in the final battle, with her identity being an incidental aspect of her established character rather than a focus for either positive affirmation or negative portrayal.

The film features multiple female characters, including Kagura, Tsukuyo, and Nobume, who are depicted engaging in and winning close-quarters physical combat against numerous male opponents using martial arts, melee weapons, and enhanced physical strength.