This film does not feature any identifiable LGBTQ+ characters or themes. The narrative is entirely focused on the established heterosexual relationships and the historical/action plotlines of the Rurouni Kenshin universe, resulting in no depiction of queer identity.

The film "Rurouni Kenshin: The Final" does not feature any identifiable transsexual characters or themes. The narrative focuses on Kenshin's past and his conflict with Enishi, without incorporating elements related to transsexual identity or experiences.

The film is a faithful adaptation of the Rurouni Kenshin manga. All major and supporting characters retain their established genders from the source material. There are no instances of characters canonically or historically established as one gender being portrayed as a different gender on screen.

The film is a Japanese live-action adaptation of a Japanese manga, featuring Japanese characters portrayed by Japanese actors. There are no instances of characters canonically established as one race being portrayed as a different race.