Mary and The Witch's Flower does not feature any discernible LGBTQ+ characters, relationships, or themes. The story focuses on a young girl's magical adventure without incorporating elements related to queer identity or experiences.

Mary and The Witch's Flower does not feature any identifiable transsexual characters or themes. The story centers on a young girl's discovery of magic and her adventures at a witch's school, with no elements pertaining to transgender identity.

The film features Mary Smith, who primarily uses magic and flight to navigate challenges and escape antagonists. While female characters are present and involved in magical conflicts, there are no instances where a female character defeats one or more male opponents in direct physical combat.

The film is an adaptation of the novel "The Little Broomstick." All major characters, including Mary, Peter, Madam Mumblechook, and Doctor Dee, retain their established genders from the source material.

The film is an adaptation of a British novel, and its characters, including protagonist Mary Smith, are depicted in a manner consistent with their implied racial background in the source material. No characters established as one race in the novel are portrayed as a different race in the film.