The film does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses exclusively on the demon slayer corps, their training, and battles against demonic threats, without incorporating elements related to queer identity or relationships.

The film does not feature any identifiable transgender characters or themes within its narrative. No characters are presented as transgender, nor are there any plot points that explore transgender identity.

The film is a direct continuation and compilation of the existing Demon Slayer anime series, which faithfully adapts the original manga. All established characters maintain their canonical genders as depicted in the source material and prior installments.

The film is an anime adaptation where all established characters, who are canonically Japanese, maintain their original racial depiction through animation. No characters established as one race are portrayed as a different race.

Not depicted in the film.