One Piece Film Red does not feature any identifiable transgender characters or themes. The story centers on Uta's musical abilities and her past, with no elements related to transsexual identity present in the plot or character portrayals.

The film introduces Uta, a new character, but all established characters from the One Piece canon maintain their original genders. No character previously established as one gender is portrayed as a different gender in this film.

This animated film features established characters whose visual depictions remain consistent with their long-standing portrayals in the source manga and anime. New characters introduced do not constitute a race swap. No existing character's race was altered.