The film does not feature any identifiable LGBTQ+ characters or themes. While one character, Mare Bello Fiore, is depicted wearing traditionally feminine clothing, this is presented as a design choice by his creator rather than an exploration of gender identity or expression. The protagonist, Ainz Ooal Gown, is an undead lich and thus lacks romantic or sexual interest, but this is a consequence of his state, not an explored identity.

The film 'Overlord: The Dark Hero' does not feature any identifiable transsexual characters or themes. The narrative focuses on fantasy adventure and power dynamics within its world, without engaging with transgender identity. For example, no character's arc involves a journey of gender transition or self-identification as transsexual.

The film is a compilation of the first season of the Overlord anime series, which faithfully adapts the characters from the original light novel series. All named characters retain their established genders from the source material without alteration.

The film adapts characters from a fantasy light novel and anime series. The main characters are either non-human fantasy creatures or human-like characters whose race was never explicitly defined in a way that would allow for a race swap.

Not depicted in the film.