The film does not contain any identifiable LGBTQ+ characters or themes. While some male characters cross-dress for disguise, this is solely a plot device for comedic effect and infiltration, not an exploration of queer identity. Therefore, the net impact on LGBTQ+ portrayal is N/A.

The film includes male characters crossdressing for tactical infiltration, handled pragmatically without stigma. This is a plot device, not an exploration of transsexual identity or themes. The movie does not explicitly portray transsexual characters or the trans community, nor does it delve into trans experiences, resulting in no identifiable trans depiction.

The film features male protagonists disguising themselves as women as a plot device. However, their canonical gender remains male, and the portrayal on screen does not alter their established gender identity from the source material. No characters established as one gender are portrayed as a different gender.

The film is an anime adaptation of a Japanese manga, faithfully portraying all characters, who are consistently depicted as ethnically Japanese, without any changes to their established race from the source material.