Based on the provided details, there is insufficient information to assess the portrayal of LGBTQ+ characters or themes in 'Deadpool & Wolverine'. Therefore, a definitive evaluation of its net impact cannot be made at this time.

Based on currently available information for 'Deadpool & Wolverine, 2024', there is insufficient data to assess the film's portrayal of transsexual characters or themes. No plot points or character arcs related to this aspect have been publicly disclosed, preventing a determination of its net impact.

The film features female characters, such as Cassandra Nova, who engage in combat. However, their victories over male opponents are primarily achieved through the use of their mutant superpowers, such as telepathy and telekinesis, rather than through direct physical combat skills like hand-to-hand fighting or melee weapon proficiency.

No established main or legacy characters, such as Deadpool or Wolverine, have their canonical gender altered in this film. While a non-binary actor portrays a canonically female character (Cassandra Nova), there is no explicit indication that the character's on-screen gender presentation has changed from female.

Based on the provided starring cast, the characters Deadpool, Wolverine, and Cassandra Nova are portrayed by actors whose races align with their established canonical depictions in source material and previous installments.