No LGBTQ+ characters or themes appear in the film. The narrative focuses on supernatural horror encountered by a group of thrill-seeking influencers, without any queer representation.

The film contains no transgender characters or themes. Urban explorers face supernatural horrors in an abandoned hotel, with the narrative focusing on survival and thrills without addressing transsexual identity.

The movie does not contain any action or adventure elements.

The adaptation retains the established genders of key characters from the source novel, such as Rick as male and Cora as female, with Diane introduced as a new addition to the explorer group without altering any canonical gender.

Do Not Enter adapts David Morrell's novel Creepers, in which main characters lack specified races or ethnicities. Casting features diverse actors, but without canonical racial baselines, no race swaps occur.