Glisten and the Merry Mission does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on traditional holiday elements, family, and community, with no explicit or implicit portrayal of the LGBTQ community within its story or character framework.

Glisten and the Merry Mission does not feature any transsexual characters or explore themes related to the transsexual community. The story centers on a young elf's quest to save Christmas, focusing on themes of belief, friendship, and the holiday spirit, without any representation of gender identity topics.

The film does not feature any scenes where female characters engage in direct physical combat with male opponents. Action sequences primarily involve animal characters or magical challenges, with no instances of female characters defeating male opponents in close-quarters combat.

The film is an original animated production, not an adaptation of pre-existing material with established character genders. Characters based on traditional figures, like Santa Claus and Donner, maintain their widely recognized genders. No characters were depicted with a gender different from their canonical or historical baseline.

The film features original characters, thus lacking prior canonical racial depictions to be swapped from. Santa Claus's portrayal aligns with his traditional depiction. Animal characters do not possess human racial attributes.