The Book of Life does not include any identifiable LGBTQ+ characters or themes. The narrative centers on a traditional love triangle and family expectations, with no explicit or implicit representation of queer identities or experiences. Therefore, the film's net impact on LGBTQ+ portrayal is N/A due to the absence of such content.

The film "The Book of Life" does not feature any identifiable transsexual characters or themes. Its narrative focuses on Mexican folklore, love, and destiny, without engaging with transgender identity in any capacity.

The Book of Life features original characters created for the film, inspired by Mexican folklore. There are no instances where a character, previously established as one gender in source material or prior adaptations, is portrayed as a different gender.

The Book of Life is an original animated film with characters created for this specific production. There is no prior source material, historical record, or previous installment to establish a character's race before this film's creation, thus precluding any race swaps.