The film does not contain any identifiable LGBTQ+ characters or themes. There are no explicit portrayals, positive or negative, of queer identity or relationships within the story.

The film "How to Train Your Dragon: The Hidden World" does not feature any identifiable transsexual characters or themes. Its narrative focuses on Hiccup's journey, dragon conservation, and the discovery of a hidden world, without engaging with gender identity topics.

The film features female characters like Astrid and Valka who are skilled dragon riders and participate in battles. However, their combat is primarily dragon-mounted or involves ranged attacks. There are no scenes depicting a female character defeating one or more male opponents in direct, close-quarters physical combat.

The film is the third installment in an animated franchise. All returning characters maintain their established genders from previous films and source material. New characters introduced in this film do not have a prior canonical gender to swap from.

All established characters in "How to Train Your Dragon: The Hidden World" maintain their visually depicted race from previous installments and the source material. No character originally established as one race is portrayed as a different race.