The Wonderful Autumn of Mickey Mouse does not include any discernible LGBTQ+ characters or themes. The narrative focuses on the established Disney characters in a family-friendly, comedic context without exploring queer identities or experiences.

The film does not feature any identifiable transsexual characters or themes, resulting in no specific portrayal to evaluate within the narrative.

The film is an animated short featuring comedic situations and character interactions. Female characters, such as Minnie Mouse and Daisy Duck, do not participate in or win close-quarters physical combat against male opponents.

This animated short features established Disney characters such as Mickey Mouse, Minnie Mouse, Donald Duck, and Goofy. All main characters are portrayed with their historically canonical genders, with no instances of a character's gender being altered from prior portrayals.

The film features established anthropomorphic animal characters like Mickey Mouse and Minnie Mouse. These characters do not possess a human race, and thus the concept of a 'race swap' does not apply to their portrayal.