Hustlers portrays a deeply intimate and loyal bond between its central female characters, Destiny and Ramona, which functions as a chosen family. This relationship, while not explicitly labeled, is strongly queer-coded and depicted with dignity, complexity, and as a source of profound emotional support, contributing to a net positive portrayal of non-traditional female love and solidarity.

The film is based on real historical figures. At least two characters, based on real-life white individuals (Samantha Barbash and Marsi Rosen), are portrayed by actresses of different races (Jennifer Lopez as Latina and Keke Palmer as Black).