Dope features LGBTQ+ characters, including a positively portrayed transgender woman, Nakia, and a main character, Jib, who expresses fluid sexuality. The film integrates these identities respectfully into the narrative, avoiding stereotypes and presenting them as accepted aspects of the characters, resulting in a net positive portrayal.

The film features Jaleel, a transgender woman portrayed with dignity and agency. While cis characters initially react with surprise to her identity, the narrative focuses on their growth and acceptance rather than degrading Jaleel. The net impact is positive, affirming her worth and challenging prejudice.