The film does not feature any identifiable transsexual characters or themes. The narrative focuses on other aspects of identity and social commentary, with no direct or indirect portrayal of transsexual individuals or experiences.

The movie does not contain any action or adventure elements.

Glass Onion is an original film with new characters, except for Benoit Blanc, whose gender remains consistent with the previous installment. No characters were established in prior canon or history with a different gender.

All characters in "Glass Onion: A Knives Out Mystery" are either original to the film or, in the case of Benoit Blanc, portrayed by the same actor as in the previous installment. No character was previously established as a different race in source material or prior canon.