Mississippi Burning (1988) does not feature any LGBTQ+ characters or themes. The film's narrative is exclusively centered on the historical events of the civil rights movement, racial prejudice, and the investigation of the murders of civil rights workers in the American South.

Mississippi Burning is a historical drama centered on the investigation of civil rights workers' murders in 1964 Mississippi. The film primarily explores themes of racial injustice and the civil rights movement. There are no identifiable transsexual characters or themes depicted in the film's narrative or character arcs.

The movie does not contain any action or adventure elements.

The film features fictional FBI agents investigating a historical event. There are no instances where a character, established as one gender in source material or history, is portrayed as a different gender.

The film features original characters (FBI agents) created for the movie, and while based on a historical event, it does not portray specific historical figures with altered races. No established character's race was changed.