The film "The Three Burials of Melquiades Estrada" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on themes of friendship, justice, and the border, without incorporating queer identities or experiences into its plot or character arcs.

The film "The Three Burials of Melquiades Estrada" does not feature any identifiable transsexual characters or themes. Its narrative focuses on themes of justice, friendship, and the U.S.-Mexico border, primarily through the experiences of its male protagonists.

The film does not portray any female characters engaging in direct physical combat against male opponents. The narrative primarily follows male characters and their journey, with female characters in supporting roles that do not involve action sequences.

The film is an original story with characters created specifically for this production. There is no prior source material, historical record, or previous adaptation from which characters' genders could have been established and subsequently changed.

The film is an original screenplay, not an adaptation of prior material or a biopic. All characters were created for this specific film, meaning there is no pre-existing canon or historical record to establish a character's race for a potential swap.