The film features Deputy Sheriff Hollis Pogue, an openly gay character portrayed with dignity, complexity, and agency. His identity and relationship are integrated respectfully into the narrative, reflecting a positive and affirming stance without being the central conflict. The depiction avoids stereotypes and contributes to a nuanced portrayal of a diverse community.

Lone Star features Pilar, a respected businesswoman whose past as a transsexual woman is revealed. The film handles this revelation with dignity and empathy, portraying Pilar as a complex character. Challenges she faced are framed as external societal pressures, affirming the worth of her identity rather than denigrating it.

The film portrays characters within a predominantly Christian community, critically examining how some individuals, like Hollis Peveto, misuse religious rhetoric to justify bigotry and racism. The narrative unequivocally condemns such prejudice, positioning the audience to reject these hateful interpretations and sympathize with those targeted, rather than condemning Christianity itself.