Josee, the Tiger and the Fish (2020) does not feature any identifiable LGBTQ+ characters or themes. The film's central narrative revolves around a heterosexual romance and the personal growth of its two main protagonists, Josee and Tsuneo, without any queer representation.

Based on available information, 'Josee, the Tiger and the Fish' does not appear to feature any transsexual characters or themes. The story centers on a romance between a disabled woman and a university student, indicating no depiction relevant to the evaluation criteria.

The movie does not contain any action or adventure elements.

The 2020 animated film adapts a short story and previous film where the main characters, Josee and Tsuneo, were established as female and male, respectively. Their genders remain consistent in this adaptation, with no significant characters undergoing a gender change from their source material.

The 2020 anime film "Josee, the Tiger and the Fish" is an adaptation of a Japanese short story and previous Japanese film. The characters, originally and consistently depicted as Japanese, are voiced by Japanese actors and portrayed as Japanese in the anime. There is no change in racial portrayal from the source material.