The film Fate/stay night: Heaven's Feel II. Lost Butterfly does not feature any identifiable LGBTQ+ characters or themes. Its narrative centers on the established heterosexual romance and dark fantasy elements, resulting in no specific portrayal of queer identities or experiences within its plot.

The film does not feature any identifiable transsexual characters or themes. Its narrative is centered on the established lore of the Holy Grail War and the personal struggles of its main cast, with no elements related to transsexual identity present in the plot or character arcs.

The film features female characters engaging in combat. However, instances where female characters defeat male opponents involve either overwhelming supernatural abilities (Servants) or do not result in a clear victory through physical skill or martial arts against a capable male opponent.

The film is a direct adaptation of the 'Heaven's Feel' route from the 'Fate/stay night' visual novel. All major and established characters retain their canonical genders as depicted in the original source material and prior adaptations.

The film is an anime adaptation of a Japanese visual novel. All major characters, whether Japanese or mythological figures, maintain their established racial or visual depictions from the source material and prior adaptations. There are no instances of a character canonically established as one race being portrayed as a different race.