The film Fate/stay night: Heaven's Feel II. Lost Butterfly does not feature any identifiable LGBTQ+ characters or themes. Its narrative centers on the established heterosexual romance and dark fantasy elements, resulting in no specific portrayal of queer identities or experiences within its plot.

The film does not feature any identifiable transsexual characters or themes. Its narrative is centered on the established lore of the Holy Grail War and the personal struggles of its main cast, with no elements related to transsexual identity present in the plot or character arcs.

The film features several female characters who engage in combat, including human mages and supernatural Servants. However, no human female character is depicted defeating a male opponent in direct physical combat using skill, strength, or martial arts. Female Servants engage male Servants, but their victories are achieved through their inherent supernatural abilities and magical weapons, rather than conventional physical contests.

The film is a direct adaptation of the 'Heaven's Feel' route from the Fate/stay night visual novel. All characters, including the female portrayal of Saber (Artoria Pendragon), maintain their established genders as depicted in the original source material and prior franchise installments, without any changes.

The film is an anime adaptation of a Japanese visual novel. All major characters, whether Japanese or mythological figures, maintain their established racial or visual depictions from the source material and prior adaptations. There are no instances of a character canonically established as one race being portrayed as a different race.