The film 'Fate/stay night: Unlimited Blade Works, 2010' does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on the Holy Grail War and the heterosexual relationships of its main protagonists, resulting in no portrayal of LGBTQ+ elements within the movie.

The film Fate/stay night: Unlimited Blade Works, 2010 does not feature any identifiable transsexual characters or themes. The narrative focuses on fantasy battles and character development within a magical setting, without incorporating elements related to transsexual identity or experiences, leading to a rating of N/A.

The film features female characters like Saber and Rin Tohsaka who engage in combat. Saber, a master swordswoman, fights male Servants but does not achieve a clear victory over them in direct physical combat within the movie's narrative. Rin primarily uses magic or fights other female characters.

The film adapts the 'Unlimited Blade Works' route of the Fate/stay night visual novel. All main characters, including Saber, retain their established genders from the original source material. There are no instances where a character canonically established as one gender is portrayed as a different gender in this adaptation.

This anime film adapts a Japanese visual novel. The characters maintain their established visual designs and implied East Asian ethnicity from the source material, with no instances of a character canonically established as one race being portrayed as a different race.