Sailor Moon S the Movie: Hearts in Ice does not feature any identifiable transsexual characters or themes. The narrative focuses on an alien antagonist and the Sailor Guardians' battle to protect Earth, without engaging with gender identity or transition in its plot or character arcs.

The film features the female Sailor Guardians battling Princess Snow Kaguya and her Snow Dancers, who are all female. There are no instances where a female character defeats one or more male opponents in close-quarters physical combat.

All major characters in the film retain their established genders from the original manga and previous anime installments. No character canonically established as one gender is portrayed as a different gender.

The 1994 animated film, an adaptation of the Japanese manga and anime series, maintains the original racial depictions of all established characters, who are consistently portrayed as Japanese. No character canonically established as one race is depicted as a different race.