The film does not feature any identifiable transsexual characters or themes. The narrative centers on the established cisgender protagonists and an alien antagonist, Fiore, whose intense bond with Mamoru is explored without any connection to gender identity or transition. No elements within the plot or character arcs suggest a transsexual portrayal.

Female characters, primarily the Sailor Guardians, engage in combat against the male antagonist, Fiore. Their victories are achieved through the use of magical energy attacks and superpowers, rather than direct physical combat, martial arts, or melee weapon skills.

All established main characters, such as the Sailor Senshi and Tuxedo Mask, retain their canonical genders. The primary antagonist, Fiore, is an original character created for this film and therefore does not have a pre-existing gender to be swapped.

The film is an animated adaptation of a Japanese manga. All established characters are depicted consistently with their original East Asian/Japanese racial portrayal from the source material. No character originally established as one race is portrayed as a different race.

Not depicted in the film.