The film 'Kuiba' does not feature any identifiable LGBTQ+ characters or themes within its narrative. The story focuses on fantasy adventure and character development without incorporating elements related to queer identity or experiences, resulting in no direct portrayal.

The film "Kuiba" does not feature any identifiable transsexual characters or themes. Its narrative is centered on a fantasy adventure, and there is no depiction or discussion of transgender identity within the story. Therefore, the net impact on the portrayal of transsexual characters and themes is N/A.

The film does not contain scenes where a female character defeats one or more male opponents in direct close-quarters physical combat through skill, strength, or martial arts. While powerful female characters exist, their combat is not depicted in a manner that aligns with the specified criteria for such feats.

The film "Kuiba" (2011) introduces original characters within its own universe. There is no prior source material, historical record, or previous installment from which character genders could have been canonically established and subsequently swapped in this adaptation.

Kuiba (2011) is an original animated film. Its characters were created for this production and do not have a prior canonical or historical racial establishment from source material or real-world history that could be altered.