Lightyear does not feature any identifiable transsexual characters or themes. While the film includes a prominent same-sex couple, this representation does not fall under the scope of transsexual depiction as defined by the rubric.

Female characters like Izzy Hawthorne and Darby Steel participate in action sequences, primarily fighting against Zurg's robots. Their victories are achieved through the use of advanced technology, gadgets, and teamwork, rather than direct close-quarters physical combat against male opponents.

The film "Lightyear" introduces new characters and provides an origin story for Buzz Lightyear, who remains male. No established characters from the "Toy Story" universe or other source material have their gender changed in this film.

Lightyear introduces the 'real' human characters that the Toy Story toys are based on. No human character from the film had a prior canonical or widely established race in previous installments or source material, as they were new depictions. Therefore, no character's race was changed from a previously established one.