Due to the absence of specific information about LGBTQ+ characters or themes in 'Mars Express, 2023', a comprehensive evaluation of its portrayal cannot be made. Therefore, the net impact on LGBTQ+ representation remains unknown.

There is insufficient information available to assess the portrayal of transsexual characters or themes in 'Mars Express, 2023'. Without details on the film's content, it is not possible to determine if such depictions exist or to provide supporting examples of character arcs or plot points.

Without access to the film's specific combat sequences or detailed plot summaries, it is not possible to determine if any female characters engage in and win direct physical combat against male opponents.

Mars Express is an original animated film, not an adaptation or reboot of pre-existing material. All characters are new creations for this specific production, therefore, no character can be considered a gender swap from prior canon.

Mars Express is an original animated film, meaning all its characters are new creations for this specific production. There is no prior source material or established canon from which characters could be race-swapped.