Micmacs features a subtly depicted gay character, Slammer, who is portrayed with dignity and complexity. His gentle nature and implied identity are presented as endearing qualities, not sources of mockery or conflict. He is an integral and respected member of the protagonist group, contributing positively to the narrative. The film embraces his unique traits within its whimsical world, resulting in a net positive portrayal.

The film features Placard, a male character who consistently presents as a woman. This character is depicted with dignity and warmth, serving as a maternal and resourceful member of the protagonist's eccentric group. The portrayal is affirming, presenting Placard's gender expression as a natural and accepted aspect of their identity, free from ridicule or negative stereotypes. Placard's unique identity is simply part of a respected individual within the ensemble.

The film strongly portrays and celebrates a 'chosen family' of misfits and outcasts as its central and most positive family unit, prioritizing these bonds over traditional biological or nuclear family structures, which are largely absent or tragic in the narrative.