The film Dofus - Book I: Julith does not feature any identifiable transsexual characters or themes. The narrative primarily focuses on the adventures of Joris and his companions in the city of Bonta, centered around a magical artifact and a powerful antagonist. No plot points or character arcs within the story depict or allude to transsexual identities.

The film features several female characters who engage in combat, including Julith, Lilotte, Bakara, and Marline. However, their combat styles primarily involve ranged attacks (archery) or magical abilities. There are no instances where a female character is depicted winning a close-quarters physical fight against one or more male opponents using hand-to-hand combat, martial arts, or melee weapons.

The film features characters from the established Dofus universe. All major and recurring characters maintain their canonical gender as depicted in the source material and prior installments. No character originally established as one gender is portrayed as a different gender in this film.

The animated film maintains the established visual characteristics and racial depictions of its characters from the Dofus universe's source material. No character canonically established as one race is portrayed as a different race in this adaptation.

Not depicted in the film.