The film features Victory, a non-binary character, who is depicted with vibrancy and dignity. The Portokalos family, particularly Aunt Voula, demonstrates acceptance and support, framing Victory's identity positively. This portrayal is highlighted as an affirming representation of queer joy and non-binary identity within a traditional family setting.

My Big Fat Greek Wedding 3 features trans characters integrated subtly and respectfully into the family narrative. The film portrays them with normalcy, avoiding stereotypes, and highlights family acceptance and love. This depiction aligns with the movie's themes of inclusivity and unconditional familial support, resulting in a net positive portrayal of trans identity.

The character Victory, originally aligned with traditional binary gender roles in the source material, is portrayed as non-binary in My Big Fat Greek Wedding 3, which constitutes a gender swap.

The film portrays Greek Orthodox Christianity primarily through its cultural traditions and strong emphasis on family values, presenting them with respect and warmth. While a detail like cremation might diverge from strict doctrine, the overall narrative affirms the positive aspects of faith-rooted community and familial love.