My Hero Academia: You're Next does not explicitly feature LGBTQ+ characters or themes. The narrative centers on established hero dynamics, power struggles, and personal growth, with no direct exploration or representation of LGBTQ+ issues based on available information.

The film 'My Hero Academia: You're Next' does not specifically focus on or detail the portrayal of transsexual characters or themes. While the broader My Hero Academia series includes transgender characters, their identifiable presence or depiction within this particular movie is not provided in the available information.

The provided information confirms the presence of active female fighters, such as Anna, who participate in combat and strategic efforts. However, it does not offer specific details or scenes where a female character is explicitly shown to defeat one or more male opponents in direct physical combat, without relying solely on superpowers or other excluded methods.

The film features existing and potentially new female characters in prominent roles, consistent with the franchise's portrayal of gender. There is no information indicating any character previously established as one gender has been portrayed as a different gender.

The provided information explicitly states there is no emphasis on racial diversity or race swapping in the narrative of "My Hero Academia: You're Next." The film continues the franchise's trend of varied characters without altering established character races.