The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on friendship, creativity, and overcoming challenges within a high school and theme park setting, without exploring queer identities or relationships.

The film "My Little Pony: Equestria Girls - Rollercoaster of Friendship" does not feature any identifiable transsexual characters or themes. The narrative focuses on friendship, magic, and high school challenges among its cisgender characters, without addressing gender identity in this specific context.

The movie does not contain any action or adventure elements.

The film features the established female main characters from the My Little Pony: Equestria Girls series, who maintain their canonical gender from the broader My Little Pony franchise. No characters previously established as male are portrayed as female, nor vice-versa.

The film features characters who originated as ponies and were given human-like forms in the Equestria Girls spin-off. Their visual depictions in this installment are consistent with their established appearances in previous Equestria Girls media, with no character's race being altered from prior human-form portrayals.