The film "Scooby-Doo! and the Legend of the Vampire" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the Mystery Inc. gang investigating supernatural occurrences at a music festival, with no elements related to queer identity or experiences present in the plot or character arcs.

The film "Scooby-Doo! and the Legend of the Vampire" does not feature any identifiable transsexual characters or themes. The narrative focuses on the Mystery Inc. gang investigating a vampire legend at a music festival in Australia, without engaging with gender identity topics.

The movie does not contain any action or adventure elements.

The film features the classic Mystery Inc. gang (Fred, Daphne, Velma, Shaggy, Scooby-Doo) who retain their long-established genders. No previously established characters from the Scooby-Doo canon or other source material have their gender altered in this installment.

The film features the classic Scooby-Doo characters—Fred, Daphne, Velma, and Shaggy—all of whom are depicted consistent with their long-established racial portrayals from the original animated series. No character's race was altered from their canonical depiction.