Scooby-Doo! and Krypto, Too! does not explicitly portray or focus on LGBTQ+ themes or characters. The narrative centers on mystery-solving and superhero action, with no notable scenes or character arcs related to LGBTQ+ identity or community. Therefore, there is no identifiable LGBTQ+ representation in the film.

Scooby-Doo! and Krypto, Too! does not portray or reference transsexual characters or themes. The narrative centers on a mystery involving the Justice League and Mystery Inc., without engaging with trans identities. While broader Scooby-Doo media has faced critiques regarding gender-related tropes, these do not apply to this specific 2023 film, resulting in no direct representation.

The movie does not contain any action or adventure elements.

All established characters from the Scooby-Doo and DC Comics universes maintain their canonical genders. Original characters created for the film do not have prior established genders to compare against, thus no gender swaps are present.

The casting for all major characters, including Fred, Daphne, Shaggy, Velma, Wonder Woman, Lex Luthor, and Lois Lane, aligns with their traditional depictions as white or European-descended characters. No instances of a character established as one race being portrayed as a different race were identified.