This Scooby-Doo animated film does not feature any discernible LGBTQ+ characters or themes. The plot centers on a traditional mystery within a superhero convention, and queer identity is not depicted or referenced in any capacity.

The film "Scooby-Doo! Mask of the Blue Falcon" does not feature any identifiable transsexual characters or themes. The narrative focuses on a mystery at a comic convention, with no elements related to transgender identity or experiences present in the plot or character arcs.

The film features female characters like Daphne Blake and Velma Dinkley, who participate in the mystery-solving. However, their roles do not involve engaging in or winning direct, close-quarters physical combat against male opponents. The villain, a female character in disguise, is apprehended through traps and deduction, not physical defeat by other female characters.

The film features the established Scooby-Doo characters and the Blue Falcon, all retaining their canonical genders from previous iterations. No legacy characters are portrayed with a different gender.

The film features the established Scooby-Doo gang and characters from the Blue Falcon universe, all of whom are depicted consistent with their original and long-standing racial portrayals. No characters who were canonically established as one race are portrayed as a different race.