The SpongeBob Movie: Sponge on the Run does not explicitly feature any identifiable LGBTQ+ characters or themes. The narrative focuses on SpongeBob's quest to rescue his pet snail, Gary, without incorporating elements related to queer identity or relationships.

The film does not feature any identifiable transsexual characters or themes within its narrative. Consequently, there is no specific portrayal or impact related to transsexual identity to evaluate, resulting in an N/A assessment.

The film does not feature any scenes where a female character engages in and wins close-quarters physical combat against one or more male opponents. While female characters like Sandy Cheeks are present and contribute to the plot, their actions do not involve direct physical defeat of male adversaries.

The film features established characters from the SpongeBob SquarePants franchise, all of whom retain their canonical genders. New characters introduced do not represent gender-swapped versions of existing ones.

The film features anthropomorphic sea creatures as its main characters, who do not possess human racial characteristics. Any human characters introduced are new or do not involve a race change from a previously established human racial identity.