Animaniacs: Wakko's Wish does not contain any discernible LGBTQ+ characters or themes. The narrative focuses on the Warner siblings' quest for a wish, with no elements related to queer identity or experiences.

The film "Animaniacs: Wakko's Wish" does not feature any identifiable transsexual characters or themes. Its narrative and character arcs do not engage with transgender identity in any capacity, resulting in no depiction to evaluate.

The film features female characters like Dot Warner who participate in chaotic, cartoon-style action. However, their victories against male opponents are achieved through exaggerated toon physics, slapstick, and comedic gags, rather than direct physical combat involving skill, strength, or martial arts.

The film features the established characters from the Animaniacs series, all of whom retain their original canonical genders. No character previously established as one gender is portrayed as a different gender.

The film features animated characters, primarily anthropomorphic animals or original human characters whose race was established within the Animaniacs animated series. There is no evidence of any character being portrayed as a different race than their original or canonical depiction.