This film, aimed at young children, does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on friendship and a magical adventure within a context that does not include queer identities or experiences.

The film "My Little Pony: Twinkle Wish Adventure" does not feature any identifiable transsexual characters or themes. The narrative focuses on the magical adventures of ponies, with no elements related to gender identity or transgender experiences.

The movie does not contain any action or adventure elements.

The film features established My Little Pony characters from the G3 era, such as Pinkie Pie and Scootaloo, all of whom maintain their historically female gender portrayals. No characters canonically established as male are depicted as female, or vice-versa.

The characters in My Little Pony: Twinkle Wish Adventure are anthropomorphic ponies, not humans. The concept of human racial categories and subsequent race swaps does not apply to these characters.