National Treasure: Book of Secrets does not feature any identifiable LGBTQ+ characters or themes. The film's plot is entirely centered on an adventure involving historical mysteries and national treasures, rendering the portrayal of LGBTQ+ elements as N/A.

The film "National Treasure: Book of Secrets" does not feature any identifiable transsexual characters or themes. Its narrative focuses on historical adventure, puzzles, and family dynamics, with no elements related to gender identity or transition. Therefore, the net impact on the portrayal of transsexual characters and themes is N/A.

The film does not depict any female characters engaging in or winning close-quarters physical combat against one or more male opponents. Female characters are present but do not participate in such combat scenarios.

The film is a direct sequel to 'National Treasure' and retains its established characters with their original genders. New characters introduced are original to this installment, and historical figures are depicted consistent with their documented genders. No instances of gender swapping are present.

This film is a sequel where all returning characters are portrayed by the same actors or actors of the same race. New characters and historical figures introduced do not contradict any established canonical or historical racial depictions.