Navajeros features a protagonist, El Jaro, who engages in a homosexual relationship alongside a heterosexual one. The film portrays his sexuality with a non-judgmental and empathetic tone, integrating it as a natural aspect of his complex character. While the narrative is tragic, the film frames the characters' struggles as external, socio-economic issues rather than condemning their queer identity. This approach aligns with an affirming portrayal.

Navajeros features La Francesa, a transsexual character who is a friend and confidante to the protagonist, El Jaro. The film integrates La Francesa into the narrative as a member of the quinqui youth's social circle. While depicting the harsh realities of their life, consistent with the film's overall tone, the portrayal avoids overt mockery or villainization, presenting the character as a person within their marginalized community.

The film portrays a deeply committed, loving family unit formed by an unmarried couple, one a criminal and the other a prostitute, who have a child. It normalizes this alternative family structure and sexual freedom within a marginalized context, without endorsing traditional marriage, gender roles, or religious values.