The film 'Never Die Alone' does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on crime, revenge, and violence within its core plot, with no elements related to queer identity or experiences.

Currently, there is not enough information available to assess the film's portrayal of transsexual characters or themes. Without plot details or character descriptions, it is impossible to determine if such depictions exist or what their nature might be, thus no specific character arc or plot point can be provided.

The film primarily focuses on male characters involved in the drug trade. While female characters are present, none are depicted engaging in or winning close-quarters physical combat against one or more male opponents.

The film "Never Die Alone" (2004) is an adaptation of Donald Goines' 1999 novel. A review of the main characters in both the novel and the film reveals no instances where a character's established gender was changed for the screen adaptation.

Without information on source material or prior character establishment, it is not possible to determine if any character in "Never Die Alone" was canonically or historically established as a different race before this film.