Not Another Teen Movie uses LGBTQ+ characters and themes predominantly for crude, stereotypical humor. Characters like Areola and Les are depicted as one-dimensional gags, reinforcing harmful stereotypes without dignity or complexity. The film's reliance on mockery and problematic portrayals results in a net negative impact on LGBTQ+ representation.

The film features the character Areola, whose gender presentation is revealed as a man in drag at the film's conclusion, serving as a comedic twist. This portrayal leverages harmful stereotypes, treating the character's identity as a source of mockery rather than offering a respectful or complex depiction.

The film portrays Christian characters, such as Areola and Malik, as naive, prudish, or stereotypical, primarily for comedic effect. Their religious adherence is often the source of humor, without offering any counterbalancing nuance or positive portrayal of the faith, thus reinforcing negative stereotypes.