The film 'Once Upon a Time in China and America' does not feature any identifiable LGBTQ+ characters or themes. Its narrative centers on martial arts, cultural adaptation, and historical events in the American West, without engaging with queer identity in any capacity.

The film 'Once Upon a Time in China and America' does not include any identifiable transsexual characters or themes within its storyline. Consequently, there is no portrayal to evaluate as positive, negative, or neutral.

The film features female characters like Aunt Thirteen and Sarah, a Native American woman. While they are present during action sequences and contribute to the plot, neither character engages in or wins direct physical combat against male opponents using martial arts, hand-to-hand techniques, or melee weapons.

This film is part of a series featuring established characters like Wong Fei-hung and Thirteenth Aunt. All major characters maintain their canonical and historically recognized genders from previous installments and source material.

The film features established characters like Wong Fei-hung, who are consistently portrayed by actors of their historically and canonically established East Asian race. New characters introduced for the American setting do not have prior canonical racial identities to be swapped.