The film 'Os Trapalhões e a Árvore da Juventude' does not feature any identifiable LGBTQ+ characters or themes. Its narrative centers on the comedic exploits of the main quartet, with no elements related to sexual orientation or gender identity present in the story.

The film "Os Trapalhões e a Árvore da Juventude" is a Brazilian comedy from 1991. Based on available plot summaries and character information, there are no identifiable transsexual characters or themes present in the narrative. Consequently, the film offers no portrayal to evaluate under the provided rubric.

The film is a comedy-adventure primarily focused on its male protagonists. There are no significant female characters depicted engaging in or winning direct physical combat against male opponents.

This film is an original story for the comedy group Os Trapalhões and does not adapt characters from pre-existing source material or historical figures. All characters are original to the film's narrative, therefore no gender swaps occur.

This film is part of a long-running Brazilian comedy franchise featuring the same core cast. The main characters are portrayed by their original actors, maintaining their established races. There is no indication of any character, canonically established as one race, being portrayed by an actor of a different race.