The film 'Puss in Boots: The Three Diablos' does not include any discernible LGBTQ+ characters or themes. Its narrative focuses on Puss's adventure with three kittens, without any elements related to queer identity or experiences.

Puss in Boots: The Three Diablos does not feature any identifiable transsexual characters or themes. The film's storyline centers on Puss's mission involving three kittens, with no elements related to transgender identity or experiences. Consequently, there is no portrayal to evaluate within the scope of this framework.

The short film does not feature any significant female characters engaging in direct physical combat against male opponents. The female kitten, Perla, participates in group efforts but does not individually defeat male adversaries in close-quarters combat.

The film introduces new characters, the Three Diablos, whose genders are established within this short. Puss in Boots maintains his established male gender. No existing characters from prior canon or source material have their gender changed.

The film features Puss in Boots, whose established portrayal remains consistent, and new original characters (The Three Diablos). No existing character with a previously established race is depicted as a different race.