Based on available reviews, synopses, and promotional materials, Osamede (2025) does not appear to feature any explicit LGBTQ+ characters, themes, or depictions. The film is consistently described as a historical-fantasy drama centered on colonial resistance and spiritual destiny.

The film 'Osamede, 2025' does not depict any transsexual characters or themes. Its narrative centers on a female heroine's journey against invaders, focusing on themes of empowerment and resistance, with no information suggesting the presence of transsexual identities or related plot points.

The movie does not contain any action or adventure elements.

Based on available information, no instances were found where a character's on-screen gender in Osamede (2025) differs from their established gender in the source material or historical record. All principal characters retain their original genders.

The film is set in the historical Benin Kingdom, where characters are inherently Edo/Bini people. All major roles are portrayed by Nigerian actors, many with Edo heritage, consistent with the historical and cultural context. No character established as one race is portrayed by an actor of a different race.