The film does not feature any explicit or implicit LGBTQ+ characters, relationships, or themes. Its narrative is centered on the conflict between Mewtwo and humanity, exploring concepts of creation and purpose, with no direct portrayal or engagement with queer identities or experiences.

The film "Pokémon the Movie: Mewtwo Strikes Back - Evolution" does not feature any identifiable transsexual characters or themes. Its narrative focuses on cloning, identity, and the conflict between original and cloned Pokémon, without touching upon gender identity or transition.

The film primarily features Pokémon battles, where human trainers command their Pokémon. No female human characters are depicted engaging in or winning direct physical combat against male opponents using hand-to-hand techniques, martial arts, or melee weapons.

The 2019 film is a CGI remake of the 1998 original. All established characters, including Ash, Misty, Brock, and Mewtwo, retain their canonical genders from the source material. No character's gender was altered from prior portrayals.

The 2019 CGI film is a faithful remake of the 1998 animated movie. All major human characters, including Ash, Misty, and Brock, retain their original visual depictions and implied racial characteristics from the source material. No character established as one race was portrayed as a different race.