Ponniyin Selvan: Part II does not feature any identifiable LGBTQ+ characters or themes. The narrative is centered on traditional romantic and political conflicts within the Chola dynasty, focusing on heterosexual relationships and historical drama without incorporating queer representation.

Ponniyin Selvan: Part II does not include or portray transsexual characters or themes. Available analyses and plot summaries focus on the historical and dramatic aspects of the Chola dynasty, without any mention of trans identities or communities within the narrative.

The film features several prominent female characters who are influential and resourceful. However, none of these characters are depicted engaging in or winning direct close-quarters physical combat against one or more male opponents using martial arts, strength, or melee weapons.

The film portrays all main characters with the same gender as established in Kalki Krishnamurthy's original novel and historical context, with no instances of gender swapping.

The film's casting predominantly features actors of Tamil and South Indian descent, aligning with the historical Chola Empire setting. While some casting choices might introduce broader Indian appeal or vary in specific ethnic features, no character's race is depicted as different from their established or historical racial category.