The animated film "Pound Puppies and the Legend of Big Paw" does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses on the adventures of puppies and their human friends, without incorporating any elements related to queer identity or experiences.

The animated film "Pound Puppies and the Legend of Big Paw" does not feature any identifiable transsexual characters or themes. Its narrative centers on the adventures of anthropomorphic dogs and their human friends, without engaging with gender identity topics.

The film features several female characters, including human girl Holly and various female Pound Puppies. However, none of these characters engage in or are depicted as victorious in direct physical combat against one or more male opponents through skill, strength, or martial arts. Combat in the film is primarily comedic or involves the puppies outsmarting their captors.

The film features established Pound Puppies characters whose genders remain consistent with prior animated series and toy lines. New characters introduced in the movie do not have a pre-existing canonical gender to swap from.

The film features anthropomorphic dog characters and human characters whose racial depictions are consistent with prior iterations of the franchise. There is no evidence of any character being established as one race in source material and then portrayed as a different race in this film.